December 8, 2024 Quickly, the SH3 site fusion protein were straight immobilized about Nunc microtiter dish (Thermo Fisher Scientific), simply by aliquoting 5 g/mL solutions (in PBS) into triplicate wells, and incubating the plates at 4C overnight
April 1, 2023 The fibroblasts were obtained from Coriell Institute for Medical Research (Camden, NJ) or established in our laboratory after informed consent was obtained
February 15, 2022 Digestion products were purified by gel extraction (Qiagen) and vectors were treated with shrimp alkaline phosphatase (Promega) to prevent self-ligation